This guide is to trigger the unlock mission.
- Yami Yugi: Pick as a starter character or reach Stage 15 if you choose Seto as a starter.
- Seto Kaiba: Pick as a starter character (or reach Stage 15 if you choose Yami Yugi as a starter).
- Joey Wheeler: Reach Stage 3.
- Tea: Reach Stage 5.
- Mai: Reach Stage 10.
- Rex: Reach Stage 21.
- Weevil: Beat Standard Duelists 100 times.
- Mako: Conduct Tribute Summons 150 times.
- Keith: Beat Legendary Duelists 50 times.
- Ishizu: Summong LIGHT-attribute monster 200 times.
- Odion: Activate Trap cards 300 times.
- Pegasus: Earn 250,000 points at Pegasus unlock event, Welcome to Toon World. Or battle him at the gate.
- Yami Bakura: Earn 200,00 points at Yami Bakura unlock event, Fear the Deck of Terror! The Destiny Board of Doom.
- Paradox Brothers: Earn 180,000 points at Paradox Brothers unlock event, Guardians of the Gate. Or Battle him at the gate.
- Yami Marik: Earn 150,000 points at Yami Marik unlock event, Attack of the Rare Hunters!
- Yugi Muto: Finish Final Duel Zone for the for the first time at Duelist Chronicles: Set Sail For the Kingdom.
- Marik Ishtar: This character is currently unavailable.
- Mokuba: Bingo Mission Event.
- Solomon Muto: Unknown.
- Arkana (Pandora): Unknown.
- Kotsuzuka: Unknown.
- Espa Roba: Unknown.
- Yami Joey: Unknown.
Note: Solomon Muto, Arkana (Pandora), Kotsuzuka (Bonz), Espa Roba, and Yami Joey are not added to the game yet.