Complete versions of each ingame map, along with indicators for which rooms all of the health ups, cartridges, and abilities are located in.
There are a total of:
- 10 Health upgrades.
- 14 Cartridges.
- 8 Abilities (2 of which are acquired from Rebba in the Nexus for having certain numbers of cartridges).
Collecting all of these will give you 100% completion. It is not necessary to fill out the map or find the secret lore rooms; only the item pickups count for completion. All of the upgrades can (and must) be acquired before entering the final area, Laboratory.